Code | CSB-MP885719HU |
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In the process of expressing the recombinant Human SEMA3G protein, mammalian cells are transfected with a DNA expression vector that carries the gene encoding the desired protein (23-782aa). The cells are then cultured to induce the expression of the intended protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Human SEMA3G protein is collected and purified from the cell lysate through affinity purification, with its purity surpassing 90%, as assessed by SDS-PAGE.
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Could you please send me the exact aa sequence including the tags?. The mentioned sequence seems to be the native one without the signaling peptide. Also could you tell me if the protein is produced like that or including the N-terminal signaling peptide and then purified after secretion?
The protein is secreted into the extracellular supernatant after expression in mammalian cells. It does not contain an N-segment signal peptide sequence. We use a secretory expression vector with a secretion signal peptide.
Full sequence of the recombinant protein:
METDTLLLWVLLLWVPGSTG(signal peptide cleavage site)DAAQPARRAVRSLHHHHHHHHHHLVPRGSRT+target protein sequence(GPSPGPSVPRLRLSYRDLLSANRSAIFLGPQGSLNLQAMYLDEYRDRLFLGGLDALYSLRLDQAWPDPREVLWPPQPGQREECVRKGRDPLTECANFVRVLQPHNRTHLLACGTGAFQPTCALITVGHRGEHVLHLEPGSVESGRGRCPHEPSRPFASTFIDGELYTGLTADFLGREAMIFRSGGPRPALRSDSDQSLLHDPRFVMAARIPENSDQDNDKVYFFFSETVPSPDGGSNHVTVSRVGRVCVNDAGGQRVLVNKWSTFLKARLVCSVPGPGGAETHFDQLEDVFLLWPKAGKSLEVYALFSTVSAVFQGFAVCVYHMADIWEVFNGPFAHRDGPQHQWGPYGGKVPFPRPGVCPSKMTAQPGRPFGSTKDYPDEVLQFARAHPLMFWPVRPRHGRPVLVKTHLAQQLHQIVVDRVEAEDGTYDVIFLGTDSGSVLKVIALQAGGSAEPEEVVLEELQVFKVPTPITEMEISVKRQMLYVGSRLGVAQLRLHQCETYGTACAECCLARDPYCAWDGASCTHYRPSLGKRRFRRQDIRHGNPALQCLGQSQEEEAVGLVAATMVYGTEHNSTFLECLPKSPQAAVRWLLQRPGDEGPDQVKTDERVLHTERGLLFRRLSRFDAGTYTCTTLEHGFSQTVVRLALVVIVASQLDNLFPPEPKPEEPPARGGLASTPPKAWYKDILQLIGFANLPRVDEYCERVWCRGTTECSGCFRSRSRGKQARGKSWAGLELGKKMKSRVHAEHNRTPREVEAT)+AAAEQKLISEEDL-
Igκ leader: METDTLLLWVLLLWVPGSTG(signal peptide cleavage site)D
10xHis: HHHHHHHHHH
Thrombin site: LVPRGS
Linker: RT
Myc-Tag: EQKLISEEDL